Crystallization and preliminary X-ray analysis of alginate lyases A1-II and A1-II′ fromSphingomonassp. A1
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چکیده
منابع مشابه
Crystallization and preliminary X-ray analysis of alginate importer from Sphingomonas sp. A1.
Sphingomonas sp. A1 directly incorporates alginate polysaccharides through a 'superchannel' comprising a pit on the cell surface, alginate-binding proteins in the periplasm and an ABC transporter (alginate importer) in the inner membrane. Alginate importer, consisting of four subunits, AlgM1, AlgM2 and two molecules of AlgS, was crystallized in the presence of the binding protein AlgQ2. Prelimi...
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Phospholipase A(1) (PLA(1)) is an enzyme that hydrolyzes phospholipids and produces 2-acyl-lysophospholipids and fatty acids. This lipolytic activity is conserved in a wide range of organisms but is carried out by a diverse set of PLA(1) enzymes. Where their function is known, PLA(1)s have been shown to act as digestive enzymes, possess central roles in membrane maintenance and remodeling, or r...
متن کاملCrystallization and preliminary X-ray analysis of the thermostable sweet protein mabinlin II.
Mabinlin II is a sweet protein with the highest known thermostablility and is isolated from the seeds of Capparis masaikai Levl. grown in south China. Two crystal forms of mabinlin II were obtained using the hanging-drop vapour-diffusion method. One of them diffracts to 2.8 A resolution and belongs to space group P2, with unit-cell parameters a = 50.16, b = 50.17, c = 76.60 A, beta = 99.6 degre...
متن کاملCrystallization and preliminary X-ray analysis of the highly thermostable sweet protein Mabinlin II.
Mabinlin II is a thermostable sweet protein isolated from the mature seeds of Capparis masaikai Levl. grown in the subtropical region of the South of China. The Mabinlin II has been crystallized and diffraction data were collected to 1.7 A resolution. The crystal belongs to space group C2 with unit cell parameters a = 80.11 A, b = 51.08 A, c = 47.34 A, beta = 122.77 degrees.
متن کاملAlginate-dependent gene expression mechanism in Sphingomonas sp. strain A1.
Sphingomonas sp. strain A1, a Gram-negative bacterium, directly incorporates alginate polysaccharide into the cytoplasm through a periplasmic alginate-binding protein-dependent ATP-binding cassette transporter. The polysaccharide is degraded to monosaccharides via the formation of oligosaccharides by endo- and exotype alginate lyases. The strain A1 proteins for alginate uptake and degradation a...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section F Structural Biology and Crystallization Communications
سال: 2005
ISSN: 1744-3091
DOI: 10.1107/s174430910500299x